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| 品牌 | absin | 貨號 | abs20019A |
|---|---|---|---|
| 規格 | 100ul | 供貨周期 | 一周 |
| 概述 | |
|---|---|
| 描述 | Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY. It also reacts with the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species. |
| 克隆類別 | Polyclonal |
| 同種亞型 | Whole IgG |
| 反應種屬 | Chicken |
| 來源宿主 | Donkey |
| 形態 | Liquid |
| 特異性 | IgY (IgG) (H+L) |
| 使用方法 | Histo-/Cyto-Chemistry:- 1:100-1:800 Flow Cytometry:- 1:100-1:800 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
| 性能 | |
|---|---|
| 標識 | Alexa Fluor 488 |
| 保存方法 | Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid for 1 year. |
| 存儲溶液 | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 0.05% Sodium Azide |
| 濃度 | 1mg/ml |
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