Rabbit Anti-IL-1 Beta Polyclonal Antibody

簡要描述：Rabbit Anti-IL-1 Beta Polyclonal Antibody WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復）

產品型號：abs120224
廠商性質：生產廠家
更新時間：2025-03-26
訪問量：1313

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藥物陽性對照抗體磷酸化抗體二抗病毒抗體標簽抗體內參抗體其它一抗

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詳細介紹

品牌	absin	供貨周期	現貨
應用領域	化工,生物產業,能源

概述
別名	Rabbit Anti-IL-1 Beta Polyclonal AntibodyIL-1 β抗體;Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta. IL-1B
宿主	Rabbit
特異性	IL-1 Beta
反應種屬	Human;Mouse;Rat;
預測反應種屬	Dog;Pig;Rabbit;Guinea Pig;
應用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復）
免疫原	KLH conjugated synthetic peptide derived from human IL-1beta:101-200/269
性能
濃度	1mg/ml
純化方法	affinity purified by Protein A
類型	Polyclonal Antibody
同種型	IgG
儲存/保存方法	Rabbit Anti-IL-1 Beta Polyclonal Antibody Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
存儲溶液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
研究領域	細胞生物免疫學糖蛋白
靶標
背景說明	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008].
細胞定位	分泌型蛋白
UniProt	P01584
實驗結果圖
	Sample: Raw246.7 (Mouse)Cell Lysate at 40 ug Primary: Anti- IL-1 beta (abs120224) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 30 kD
	Tissue/cell: Human cervical carcinoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
	Blank control (black line): A431(black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary antibody: Rabbit Anti-PTP1B antibody ;Dilution: 1μg /10^6 cells; Isotype Control antibody: Rabbit IgG . Secondary antibody: Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
溫馨提示：本產品僅作科研實驗使用，不支持臨床等研究

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Rabbit Anti-IL-1 Beta Polyclonal Antibody

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