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詳細介紹
| 品牌 | absin | 貨號 | abs00840 |
|---|---|---|---|
| 規格 | 500IU | 供貨周期 | 一周 |
| 概述 | |
|---|---|
| 形態 | Lyophilized from a 0.2 μm filtered concentrated solution in sodium citrate buffer (1 liter of ddH2O containing 5.9 g of sodium citrate, 5.8 g of sodium chloride and 0.06 g of citric acid). |
| 來源 | CHO |
| 氨基酸序列 | APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR |
| 內毒素水平 | Less than 0.01 EU/μg of Recombinant HumanEPO-α as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and is no less than 1.5 × 105 IU/mg. |
| Accession # | P01588 |
| 預期分子量 | Mature human EPO, containing 166 amino acid residues, has a predicted molecular mass of approximay 21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 36-40 kDa in SDS-PAGE. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 98 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 2056 |
| 參考文獻 | |
|---|---|
| 1. Hanicki Z. 1988. Pol Arch Med Wewn, 80: 290-3.<br/>2. Haniu M, Narhi LO, Arakawa T, et al. 1993. Protein Sci, 2: 1441-51.<br/>3. Baranowska-Daca EandKsiazek A. 1994. Ann Univ Mariae Curie Sklodowska Med, 48 Suppl 3: 51-60.<br/>4. Chow KM, Szeto CC, Li PK. 2003. Am J Kidney Dis, 41: 266-7; author reply 7.<br/>5. Maurer MH, Schabitz WR, Schneider A. 2008. Curr Med Chem, 15: 1407-11. | |
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